Reporter

Part:BBa_K2607002:Design

Designed by: Valdi Ven Japranata   Group: iGEM18_UI_Indonesia   (2018-09-25)
Revision as of 16:34, 12 October 2018 by Valdiven (Talk | contribs) (→‎Source)


Improved Blue Fluorescent Protein (BFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is assembled as improvement of B0034-Blue Florescent Protein (BFP) (BBa_K592024). Our established part consists of these following features:

  • lacI regulated promoter (BBa_R0010) in the upstream
  • Double terminator (BBa_B0010 and BBa_B0012) in the downstream
  • SalI restriction site between the promoter and ribosome binding site
  • NdeI restriction site between the ribosome binding site and coding sequence
  • Modified blue fluorescence protein coding sequence to eliminate SalI restriction site in the middle without altering amino acid sequence

Source

We constructed this part with help of SnapGene and references from several previous parts (e.g. BBa_R0010, BBa_B0010, BBa_B0012, BBa_K592100, and BBa_K592024). We then ordered the final sequence from Integrated DNA Technologies, Inc. (IDT) and tested out this newly-assembled part compared with BBa_K592024 from iGEM 2018 Distribution Kit Plate 1 (well 17C).

References