Composite

Part:BBa_K2718022

Designed by: Dimitri Popoff Emeline Lemarie   Group: iGEM18_Aix-Marseille   (2018-08-27)
Revision as of 14:16, 12 October 2018 by LISM JS (Talk | contribs)


IPTG inducible promoter with RBS Endochitinase 6-His

Usage and Biology

To verify the functionallity of this part we measured chitinase production after induction of E. coli (DH5a) at OD 0.8 with 1mm IPTG for 3 hours at 37°C.

Fig 1. (left) SDS-PAGE of chitinase production and (right) western blot revealed with anti-his antibody in NI (nor induced) and I (induced) E.coli cells.
This clearly shows that we are able to produce significant amounts of chitinase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None