Part:BBa_K2671420
TetR-TetP-GroES
The gene coding for the chaperone GroES (homologue to HSP10 in eukaryotes) that is found in E.coli. It often works in conjunction to GroEL creating an environment well suited for the folding of proteins. NOTE: The sequence is codon optimized (E.coli) with IDT's tool. This sequence will not yield any results with BLASTn, but will hit GroES with BLASTx. The TetP also has one TetR binding site mutated (one base).
The gene coding for GroES is placed downstream from a tetracycline promotor. Further upstream the tetracycline repressor protein (TetR) is found, expressed by a constitutive promotor. The TetR protein has a termination sequence directly downstream from it, while the GroES gene has its transcription stop by the E.coli his operon termination sequence that is present in most pSB vectors, directly downstream of the insert.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |