Coding
BcsZ

Part:BBa_K2559000

Designed by: Yinpin Huang   Group: iGEM18_SCAU-China   (2018-10-07)
Revision as of 07:22, 12 October 2018 by Weiteng (Talk | contribs)


Bacterial cellulose synthase Z (BcsZ) The BBa_K2559000 is an endoglucanase (Bcs Z) involved in the process of cellulose hydrolysis, and its roles in cellulose biosynthesis have long remained obscure.

Usage and Biology

Bacterial cellulose synthase Z (BcsZ) from several bacteria have been shown to possess an endo-β-1,4-glucanase (cellulase) activity, and E. coli protein was even crystallized in a complex with the substrate cellopentaose, however, and the roles in cellulose biosynthesis have long remained obscure. We suspect that the BcsZ and BlgX may help the cell form the correct cellulose configuration through selective digestion.We introduced the Acetobacter xylinum bacterial cellulose synthase gene (including BcsZ, H, A, B, C, D, LX) into the model organism Synechocystis, the part is facilitate the in-depth research for other teams.

Figure. 1 Enzymatic reaction catalyzed by PSY

Transcriptional activity of PSY

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 252


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Categories
Parameters
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