Composite

Part:BBa_K2789001:Design

Designed by: Du liaoqi   Group: iGEM18_WHU-China   (2018-10-05)
Revision as of 05:42, 12 October 2018 by Duliaoqi (Talk | contribs) (→‎References)


lac operator+rbs+Ccas+terminator+Ccar+lac operator+rbs+terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1393
    Illegal BamHI site found at 2973
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 798
    Illegal AgeI site found at 1803
    Illegal AgeI site found at 1998
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have optimized the code in the Ccas and Ccar. And we deleted the EcoRI restriction site in the Ccas for future DNA ligation and digestion.


Source

All the parts come from igem registry resources.

Ccas: BBa_K608102

Ccar: BBa_K592002

Lac operator: BBa_C0010, BBa_C0012

double terminator: BBa_B0015

rbs: BBa_B0034

References

We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102)

And this part BBa_K2789001 is consists of lac operator , Ccas and Ccar (The downstream signal transducers to control gene expression)