Coding

Part:BBa_K2683003:Design

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-07)
Revision as of 03:21, 12 October 2018 by Kristiturton (Talk | contribs) (→‎References)


MS2 Protein with Polyarginine Peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A polyarginine cell penetrating peptide was chosen due to the non-specific nature of the peptide. Available research on cell penetrating peptides for bacterial systems tend to be specific for the peptidoglycan of the bacterial cell wall. Therefore, using these types of peptides would need to be expressed in a different model system as it would likely kill the E.coli we wished to express the capsid in.


Source

The MS2 capsid is originally from the MS2 bacteriphage. Our sequence was synthesized as a g-block

References

[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664

[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138