Composite

Part:BBa_K2683027

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-10)
Revision as of 01:36, 12 October 2018 by Kristiturton (Talk | contribs)

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Elongation Factor- Thermo Stable (EF-Ts) in pSB1C3

Elongation Factor- Thermo stable (EF-Ts) is a prokaryotic elongation factor that acts at the guanine nucleotide exchange factor for EF-Tu, catalyzing the release of GDP from EF-Tu. This enables EF-Tu to bind to a new GTP and catalyze another aminoacyl tRNA addition to the peptide chain [1]. This par is codon optimized for use in Escherichia coli , has a C-terminal His tag for purification purpose and has a T7 promoter, medium strength RBS and double terminator. This part was originally designed by the Lethbridge iGEM in 2017 (http://2017.igem.org/Team:Lethbridge). This part was initially characterized in the pJET plasmid (https://parts.igem.org/Part:BBa_K2443028).

This part was improved by placing the part into the pSB1C3 plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 88
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None