Composite

Part:BBa_K2683019:Design

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-10)
Revision as of 23:46, 11 October 2018 by Kristiturton (Talk | contribs) (→‎References)

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Lysine tRNA Synthetase (LysRS) in pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1459
    Illegal NgoMIV site found at 1531
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 733


Design Notes

The subparts and coding sequence of this part is codon optimized for use in E.coli .

Source

https://parts.igem.org/Part:BBa_K2443012

References

[1] Friest, W and Gauss, D. (1995) Lysyl-tRNA Synthetase. Biological Chemistry Hoppe Seyler. 377, 399-402