![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2683018
Designed by: Kristi Turton Group: iGEM18_Lethbridge (2018-10-10)
Revision as of 23:25, 11 October 2018 by Kristiturton (Talk | contribs)
Cysteine tRNA synthetase (CysRS) in pSB1C3
Cysteine tRNA synthetase is a protein that has the function of attaching cysteine onto its tRNA to form an aminoacyl-tRNA. The part is Codon optimized for use in Escherichia coli, contains a C-terminal hexahistidine tag with a serine glycine linker, has T7 Promoter, medium RBS and double terminator. These original designs were done by the 2017 Lethbridge iGEM team (http://2017.igem.org/Team:Lethbridge) This part was improved by the 2018 iGEM team by placing this composite part into the pSB1C3 standard.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1065
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 931
[edit]
Categories
Parameters
None |