Composite

Part:BBa_K2788011:Design

Designed by: Mingyue Luo   Group: iGEM18_SZU-China   (2018-10-05)
Revision as of 21:34, 11 October 2018 by M Orz (Talk | contribs) (→‎References)

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PgpdA-Bbchit-TtrpC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 903
    Illegal NotI site found at 2890
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 137
    Illegal BamHI site found at 3365
    Illegal XhoI site found at 693
    Illegal XhoI site found at 1075
    Illegal XhoI site found at 2877
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2559
    Illegal NgoMIV site found at 3088
    Illegal NgoMIV site found at 4001
    Illegal AgeI site found at 1282
    Illegal AgeI site found at 1430
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1109
    Illegal BsaI.rc site found at 3715
    Illegal SapI site found at 315


Design Notes

In order to facilitate the replacement of the gene of interest, we added HindIII and KpnI restriction sites on both sides of the gene of interest.


Source

PgpdA comes from BBa_K2040101 Bbchit comes from BBa_K2788001 TtrpC comes from BBa_K2040102

References

[1]Kan Zhuo, Li Yu, Jinling Liao. Paecilomyces lilacinus MD1 chitinase activity assay [J] Agricultural Sciences, 2009 (05): 167-170.

[2]Xiaozhen Shi, Min Wang, Huaping Huang, Lijia Guo, Wei Song, Junsheng Huang. Analysis of Chitinase Activity of Metarhizium anisopliae and Its Correlation with Toxicity of Coleoptera[J]. Guangxi Agricultural Sciences,2008(03):313-316.