Coding

Part:BBa_M31234:Design

Designed by: Kate Broadbent   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2008-05-13)
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Calcium channel-apoaequorin fusion


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1717
    Illegal BamHI site found at 964
    Illegal BamHI site found at 1614
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 485
    Illegal BsaI.rc site found at 734


Design Notes

Designing an appropriate linker


Source

Accession number for Homo sapiens neuronal voltage gated calcium channel gamma-3 subunit = AF100346

Accession number for Aequorea victoria apoaequorin = E14214

References