![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2557051
Designed by: Ge JT Group: iGEM18_NAU-CHINA (2018-10-11)
EF1α promoter-TetR
TetR is a repressor that binds to TetO sequences and inhibits the expression of downstream genes. iGEM17_Oxford inserted a TEV cleavage site in TetR (BBa_K2450202), so TetR can be recognized and cleaved by TEV protease, losing binding function and releasing inhibition of downstream genes. In our system, we use this TetR as a negative regulatory protein of the recombinase. In order to increase TetR expression to enhance repression, we combined TetR and EF1α promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 581
[edit]
Categories
Parameters
//chassis/eukaryote
None |