![](https://parts.igem.org/images/partbypart/icon_rna.png)
RNA
PORCN gRNA
Part:BBa_K2591020
Designed by: Luo Kevin Group: iGEM18_SUSTech_Shenzhen (2018-10-07)
A gRNA designed to PORCN, an essential gene to secretion of the canonical wnt protein
Usage and Biology
Single-gRNA is a small RNA which help CRISRR-Cas protein family to target the exogenous sequence, and assist the prokaryotes to defend phages. Nowadays, the artificial gRNA can achieve the modification casually in the gene level and is a excellent way to introduce mutation. In our project, we design this gRNA for porcupine O-acyltransferase (PORCN), an essential enzyme to function at the Wnt3A protein secretion. Therefore, we use the gRNA to target PORCN, we can inhibit the donor cell to secret the Wnt3A protein normally.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |