Coding
Part:BBa_K2818000
Designed by: Danny Teo Shun Xiang Group: iGEM18_NTU-Singapore (2018-10-07)
Revision as of 14:20, 11 October 2018 by Dannyteo92 (Talk | contribs)
∆RuvCIII-2 ∆HNH ∆REC2 4QC_HNHpK Sp-dCas9
dCas9 is a catalytically inactive Cas9, which still retains it's ability to bind to DNA. This truncated Sp-dCas9 has been improved upon to increase DNA binding affinity. It can be fused to an effector protein to achieve targetting to specific locus. This is an improvement from part Part:BBa_K2316001
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 251
Illegal BglII site found at 804
Illegal BamHI site found at 1622
Illegal XhoI site found at 2608 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1996
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2142
Illegal BsaI site found at 2804
Illegal BsaI.rc site found at 1027
[edit]
Categories
Parameters
//cds/enzyme
None |