Reporter
NG3

Part:BBa_K2623023

Designed by: Jiyang Zheng   Group: iGEM18_XMU-China   (2018-10-02)
Revision as of 13:04, 11 October 2018 by Hyydfl2 (Talk | contribs)


Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini

It’s another form of the BBa_K2623021 designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). The only difference is that we inserted SpyTag to C-termini of OmpA protein(instead of the N-termi in BBa_K2623021). Through the fluorescence intensity of GFP, we can evaluate the efficiency of their transport to the outer membrane and OMVs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None