Reporter
NG1

Part:BBa_K2623021

Designed by: Niangui Cai   Group: iGEM18_XMU-China   (2018-10-02)
Revision as of 12:53, 11 October 2018 by Hyydfl2 (Talk | contribs)


Bacterial outer membrane protein A (OmpA) fused with SpyTag at its N-termini and GFP at its C-termin

This part was designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We inserted SpyTag to its N-termini and SpyCather to L7Ae (BBa_K2623006) in order to anchore L7Ae to outer membrane through the bioconjugation of the SpyTag/SpyCatcher system. Through the fluorescence of GFP, we can evaluate the efficiency of their transport to the outer membrane and OMVs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1302


[edit]
Categories
Parameters
emission510 nm
excitation478 nm