Coding

Part:BBa_K2665015

Designed by: You Tong   Group: iGEM18_Kyoto   (2018-10-08)
Revision as of 10:07, 11 October 2018 by Nanbu (Talk | contribs)


TDH3-AVP1-6xHis-CYC This gene codes Arabidopsis vacuolar proton-pumping pyrophos- phatase (H+-PPase) gene (AVP1). AVP1 uses energy derived from the hydrolysis of cytosolic inorganic pyrophosphate (PPi) to orthophosphate (Pi) to actively pump protons into vacuoles. This increases acidification in vacuole, which establishes a difference in electrochemical potential for H+ across the tonoplast. The difference is used by other vacuolar transporters, such as Na+/H+ antiporters. By this process, transgenic plants overexpressing AVP1 can facilitate sequestration of Na+ into vacuole from cytoplasm and survive under high salt conditions

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1341
    Illegal BglII site found at 2598
    Illegal BamHI site found at 2394
    Illegal BamHI site found at 3030
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2690
    Illegal BsaI.rc site found at 2880


[edit]
Categories
Parameters
None