RNA

Part:BBa_K2889000:Experience

Designed by: Rui Zhou   Group: iGEM18_Worldshaper-Wuhan   (2018-10-04)
Revision as of 08:01, 11 October 2018 by Ruizhou (Talk | contribs) (→‎Applications of BBa_K2889000)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2889000

1.cell migration 1.1 Cell migration is a central process in the development and maintenance of tumor. We cloned the full length of IL7-AS-S2 into PCDNA3.1 and transfected the plasmid to 786-O cells.Through in vitro scratch wound healing assay, overexpression of IL7-AS-S2 promoted cell migration of 786-O cells.Overexpression of IL7-AS-S2 increased the migration of 786-O cells (Fig. 1 and 2). These results suggested that IL7-AS-S2 contain key structural domains.

IL7-AS-AS2.jpg


IL7-AS-S2-cell_migration.jpg


1.2 In order to investigate whether cell migration induced by IL7-AS-S2 depends on the amount of IL7-AS-S2 transfection. We transfected the different concentration of IL7-AS-S2 () into 786-0 cells.(Fig. 3 and 4)


Different_concentration_of_IL7-AS-S2.jpg


Different_concentration_of_IL7-AS-S2-.jpg

User Reviews

UNIQf504ce71e5828ae7-partinfo-00000000-QINU UNIQf504ce71e5828ae7-partinfo-00000001-QINU