Coding

Part:BBa_K2685006:Design

Designed by: LIN, WENXIN   Group: iGEM18_TUST_China   (2018-10-06)
Revision as of 07:23, 11 October 2018 by PandaLIN (Talk | contribs) (→‎Source)


TetO+TetA

This parts can provide tetracycline tolerance to our constructive detecting chasis which including tetA and tetO genes.

TetA encodes the tetracycline efflux pump protein, which selectively transports tetracycline from thecytoplasm to the periplasm in exchange for protons, thus limiting tetracycline's access to ribosomes within cells.They are proton power dependent, single polypeptide, drug-specific efflux pump, and it belong to the main promoting factor superfamily.

We also design some single sites mutation comparing with the original sequence that we have got from the biobrick bank.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 897
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 503
    Illegal NgoMIV site found at 871
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

detecting tetracycline and pump out the tetracycline


Source

TetA is PCR from the biobrick which included in the kit plate.

References