Deleted
Sample Discontinued
Experience: None
Not Used
Get This Part
RNA

Part:BBa_K2591023

Designed by: Luo Kevin   Group: iGEM18_SUSTech_Shenzhen   (2018-10-11)
Revision as of 06:44, 11 October 2018 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


a no functional gRNA which can not affect the secretion of the Wnt3A protein

Single-gRNA is a small RNA which help CRISRR-Cas protein family to target the exogenous sequence, and assist the prokaryotes to defend phages. Nowadays, the artificial gRNA can achieve the modification casually in the gene level and is a excellent way to introduce mutation. In our project, we design this gRNA to a nonsense sequence, and this gRNA have no effect on normal activity of the cell, let alone the secretion of the Wnt3A protein. We design this gRNA as a negative control group in our project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None