Part:BBa_K2539300

AlcR Expression Construct AlcR is a gene found in Aspergillus nidulans which encodes for a regulatory protein (Panozzo et al., 1997). AlcR can bind to the alcA and aldA promoters (PalcA and PaldA, respectively). In the presence both AlcR and ethanol or threonine, genes downstream of these promoters are expressed (Felenbok et al., 1988).

Construct Design LINK

This construct constitutively express alcR, a regulatory protein necessary for the activation of PalcA. We use a strong promoter and strong RBS combination (BBa_K880005) to maximize protein production, the alcR sequence (BBa_K2092001), and a double terminator (BBa_B0015) to end transcription.

PCR Check Results The part was confirmed by PCR using the primers VF2 and VR, as well as sequencing by Tri-I Biotech.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2611
  Illegal BamHI site found at 1615
  Illegal BamHI site found at 2353
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1319
  Illegal AgeI site found at 1447
  Illegal AgeI site found at 1978
  
• 1000
  COMPATIBLE WITH RFC[1000]