Coding

Part:BBa_K2692001

Designed by: Kristi Turton   Group: iGEM18_Lethbridge_HS   (2018-10-08)
Revision as of 02:46, 11 October 2018 by Kristiturton (Talk | contribs)

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Major T4 Capsid Protein gp23

gp23 is one of the two major capsid proteins that make up the head of the T4 bacteriophage. In order for the protein to fold into its proper structure, gp23 needs the help of a chaperone protein gp31 [1], which was also designed by the team this year (https://parts.igem.org/Part:BBa_K2692003; https://parts.igem.org/Part:BBa_K2692006). The gp23 protein makes up a majority of the capsid, whereby it self assembles into 160 hexamers. This part has been codon optimized for use in Escherichia coli .

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
    Illegal NotI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal BamHI site found at 1069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1118


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Categories
Parameters
None