Coding

Part:BBa_K2692002:Design

Designed by: Kristi Turton   Group: iGEM18_Lethbridge_HS   (2018-10-08)
Revision as of 02:41, 11 October 2018 by Kristiturton (Talk | contribs) (→‎Source)


Major T4 Capsid Protein gp24


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1577
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1578
    Illegal PstI site found at 1592
    Illegal NotI site found at 1585
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 753
    Illegal BamHI site found at 1248
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1578
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1578
    Illegal PstI site found at 1592
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The addition of a his tag was considered for purification purposes


Source

This part was synthesized as a g-block through IDT.

References

[1] Olson N., Gingery, M., Eiserling, F., and Baker, T. (2001) The structure of isometric capsids of bacteriphage T4. Virology. 279,385-391