Composite

Part:BBa_K2739009

Designed by: Lok In Lo   Group: iGEM18_Edinburgh_OG   (2018-10-09)
Revision as of 22:44, 10 October 2018 by Owen-lo 923 (Talk | contribs)

Hybrid promoter-PhaCAB-Bktb

This is a composite part that was created to allow us to test the functionality of BktB. PHA operon is known to produce bioplastic PHB. The coexpression of the BktB allow the production of copolymer, PHBV.

Usage and Biology

The BktB was isolated from R. eutropha H16 and being recognised as a phaA paralogous, which allows the formation of 3-ketovaleryl-CoA and leads to PHBV production. In order to enhance the 3HV fraction in PHBV, paralog bktB was introduced into E. coli BL21 (DE3) with co-expression of phaCAB operon from Ralstonia eutropha.

T--Edinburgh_OG_BBa_K2739009--image_1.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 979
    Illegal BglII site found at 1804
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 285
    Illegal NgoMIV site found at 356
    Illegal NgoMIV site found at 956
    Illegal NgoMIV site found at 1268
    Illegal NgoMIV site found at 1547
    Illegal NgoMIV site found at 2199
    Illegal NgoMIV site found at 2221
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4065
    Illegal BsaI site found at 5108


[edit]
Categories
Parameters
None