Composite

Part:BBa_K2739009

Designed by: Lok In Lo   Group: iGEM18_Edinburgh_OG   (2018-10-09)
Revision as of 22:27, 10 October 2018 by Owen-lo 923 (Talk | contribs)


Hybrid promoter-PhaCAB-Bktb

This composite part is designed to investigate the effect of PHBV production using PHA synthetic pathway (PHA operon) with BtkB co-expressed. The BktB was isolated from R. eutropha H16 and being recognised as a phaA paralogous, which allows the formation of 3-ketovaleryl-CoA and leads to PHBV production.



Usage and Biology

In order to enhance the 3HV fraction in PHBV, paralog bktB was introduced into E. coli BL21 (DE3) with co-expression of phaCAB operon from Ralstonia eutropha.

T--Edinburgh_OG_BBa_K2739009--image_1.jpg

<img src="T--Edinburgh_OG_BBa_K2739009--image_1.jpg" align="left" width="600px"/> 

the effects on PHBV production of having only phaA, only bktBand both two genes

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 979
    Illegal BglII site found at 1804
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 285
    Illegal NgoMIV site found at 356
    Illegal NgoMIV site found at 956
    Illegal NgoMIV site found at 1268
    Illegal NgoMIV site found at 1547
    Illegal NgoMIV site found at 2199
    Illegal NgoMIV site found at 2221
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4065
    Illegal BsaI site found at 5108


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Categories
Parameters
None