Coding

Part:BBa_K2739008

Designed by: Lok In Lo   Group: iGEM18_Edinburgh_OG   (2018-10-09)
Revision as of 21:45, 10 October 2018 by Owen-lo 923 (Talk | contribs)


Bktb

Btkb is paralogous to the phaA, which is involved in the bioplastic Polyhydroxyalkanoate (PHA) synthetic pathway. Its main function is to form the 3-ketovaleryl-CoA through condensation of two moleculesof acetyl-CoA.


Biology and Usage

The bktB was isolated from R. eutropha H16. 

as the most important paralogous gene for PHBV production since it showed higher substrate specificity to the C5 monomer and used 3-ketovaleryl-CoA more efficiently (Mifune et al 2010).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 831


[edit]
Categories
Parameters
None