Composite
Part:BBa_K2859001:Design
Designed by: Kyuhee Jo Group: iGEM18_HAFS (2018-10-10)
Insulin secretion (Moderate)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Insulin is normally made out of two chains. However, this can be hazardous considering the possibility of the protein folding going wrong. Hence, we used sequence of SCI-57. Secondly, the penetratin, a type of cell penetrating peptide, assists the delivery of insulin to blood vessels. Lastly, the FLAG tag allows the users to detect the insulin secretion easily using techniques such as western blotting.
Source
The SCI-57 is derived from Human Insulin