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Composite
Part:BBa_K2859000:Design
Designed by: Kyuhee Jo Group: iGEM18_HAFS (2018-10-10)
Insulin secretion (Strong)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Insulin is normally made out of two chains. However, this can be hazardous considering the possibility of the protein folding going wrong. Hence, we used sequence of SCI-57. Secondly, the penetratin, a type of cell penetrating peptide, assists the delivery of insulin to blood vessels. Lastly, the FLAG tag allows the users to detect the insulin secretion easily using techniques such as western blotting.
Source
The sequence is derived from Human Insulin.