Reporter

Part:BBa_K2610041

Designed by: Charlotte de Ceuninck van Capelle, Paula Regal   Group: iGEM18_Leiden   (2018-10-10)
Revision as of 11:51, 10 October 2018 by Chardeceuninck (Talk | contribs)


pGapA-AmilCP

Promoter GapA is naturally occurring constitutive promoter in Escherichia coli.

Usage and Biology

We, iGEM Leiden 2018, have combined this naturally occurring constitutive promoter (BBa_K2610040) from Escherichia coli with the blue chromoprotein AmilCP (BBa_K592009) in order for us to compare it to our novel stress promoter+chromoprotein constructs. GapA is known for its high constitutive expression, as is demonstrated in Figure 1.

Moreover, GapA being a natural occurring promoter there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors.

T--Leiden--gapaamilcp.jpeg

Figure 1. Constitutively expressed AmilCP

References

Munjal, N., Jawed, K., Wajid, S., & Yazdani, S. S. (2015). A constitutive expression system for cellulase secretion in Escherichia coli and its use in bioethanol production. PloS one, 10(3), e0119917.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 140


[edit]
Categories
Parameters
None