Part:BBa_K2666008

LL-37 with L.jensenii specific strong promoter RpsU

I. Part BBa K2666001 : Function

The Montpellier iGEM team 2018 designed a construction that produce RFP in Bacillus subtilis (http://2018.igem.org/Team:Montpellier/Toolbox). For that, we used the promoter R0, a B.subtilis specific strong promoter [1]. Figure 1 illustrates the detailed design.

T--Montpellier--R0-RFP_design_mtp.PNG Figure 1 : Construct design. The sequence that encode RFP fusionned with a strong promoter R0.

The objective is to check the strength of several construction in E.coli, L.jensenii & B.subtilis.

II. Proof of function

III. Design Considerations

We added spacers to all of our constructions to unable easier use of the sequence and separation of the different genes of the sequences. We used two Terminators to our sequences :BBa_B0014 & BBa_B0015 to ensure the stopping of the transcription.

Reference:

[1] Guiziou, Sarah, et al. "A part toolbox to tune genetic expression in Bacillus subtilis." Nucleic acids research 44.15 (2016): 7495-7508.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 62
  Illegal BamHI site found at 194
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]