Composite

Part:BBa_I725099:Design

Designed by: Wayne Materi   Group: iGEM07_Alberta   (2007-10-24)
Revision as of 21:59, 2 May 2008 by Scmohr (Talk | contribs) (→‎Source)

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Partial operon for butanoate pathway


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 308
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 763
    Illegal AgeI site found at 1077
    Illegal AgeI site found at 1208
    Illegal AgeI site found at 1988
    Illegal AgeI site found at 3091
    Illegal AgeI site found at 3493
    Illegal AgeI site found at 4717
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We were unable to complete addition of the fifth gene to the operon (Butanol dehydrogenase). It needs to be cloned from I725025 to complete this.


Source

Genes were synthesized from E. coli-optimized Clostridium acetobutylicum sequences from KEGG.

References