Part:BBa_K2610031
pSoxS-GFP
This composite part features the regulatory part promoter SoxS (BBa_K2610030) and the fluorescent protein GFP (BBa_E0040). It can be used to visualize upregulation of SoxS as a result of superoxide stress.
Regulatory protein SoxS is involved in the superoxide pathway in Escherichia coli. It acts a superactivator of downstream stress genes.
Usage and Biology
We, iGEM Leiden 2018, have designed this composite part as part of our project Fifty Shades of Stress. This reporter part allowed us to detect stress-induced changes in SoxS transcription using flow cytometry.
As can be seen in figure 1, treatment with increasing concentrations of nalidixic acid leads to an increasing median fluorescence. Moreover, we have integrated this promoter into a heatmap with many stress-related promoters (Figure 2). SoxS is activated specifically by nalidixic acid and hydrogen peroxide.
Figure 2. Median Fluorescence Intensity (MFI) in AU after 4 hour incubation with nalidixic acid in various concentrations.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 947
| None |