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Part:BBa_K2872881
Designed by: Dina Altarawneh Group: iGEM18_CMUQ (2018-10-10)
Template DNA sequence coding for HBB rs334 mutation (A to T) in humans, PAM site added
This is the activating sequence of Cas12a. Upon gRNA-directed binding of Cas12a to this sequence, there is an indiscriminate degradation of DNA by Cas12a
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 178
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Categories
Parameters
None |