Part:BBa_K2570002
TyrB
Phenyllactic acid（PLA）was widely found in kimchi, honey and other foods, which is a new type of natural antibacterial substance and preservative. It can inhibit a series of Gram-negative, positive bacteria and fungi, and has obvious improvement and protection to the cardiovascular system. The role has been widely used in the food and pharmaceutical industries. Our team introduced D-lactate dehydrogenase BBa_K257000 and phenylalanine aminotransferase BBa_K2570002 and expressed rocG BBa_K2570004 to introduce the cofactor circulatory system to optimize the transformation system conditions and use phenylalanine as a substrate. Production of PLA. This sequence codes for Tyrb, one of the two key enzymes in the phenyllactic acid anabolic pathway, phenylalanine aminotransferase, phenylalanine aminotransferase (PheATs, EC 2.6.1.57), also known as aromatic transaminase (AroATs) or tyrosine aminotransferase (TyrATs) , an enzyme dependent on pWe chose phenylalanine aminotransferase from Escherichia coli 21B to deamination of the substrate phenylalanine under the action of aminotransferase to produce PLA. Yridoxa-l-5'-phosphate (PLP), is a key enzyme in the biosynthesis of phenylalanine and tyrosine.
The phenylalanine aminotransferase catalyzes the conversion of the substrate phenylalanine to produce more intermediate metabolite phenylpyruvate, so that sufficient phenylpyruvate is utilized by D-lactate dehydrogenase. The product D-PLA is produced under the catalysis of D-lactate dehydrogenase BBa_K2570003.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1054
- 1000COMPATIBLE WITH RFC[1000]
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