Composite

Part:BBa_K2541405

Designed by: Shuting Zheng   Group: iGEM18_Jilin_China   (2018-10-05)
Revision as of 03:53, 10 October 2018 by Shuting (Talk | contribs)


Heat-inducible RNA-based thermosensor measurement device

A RNA thermosensor that can be used for temperature sensitive post-transcriptional regulation which is based on the change of RNA sencondary structure. The heat-inducible RNA thermosensors can initiate translation of downstream genes at high temperatures. The composite part is composed of promoter BBa_J23104, heat-inducible RNA thermosensor-51 BBa_K2541051, reporetr protein sfGFP BBa_K2541400, terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None