Coding

Part:BBa_K2570016:Design

Designed by: Xueyi Yang   Group: iGEM18_FJNU-China   (2018-09-30)
Revision as of 03:29, 10 October 2018 by Junru (Talk | contribs)


hns


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 154


Design Notes

H-NS is a global-regulated protein which could bind to genome and specifically inhibit proU. We did site-directed mutation for hns in order to meet the requirements of RFC10. Detailed information in BBa_K2570011


Source

E.coli K12.

References

[1]Interactions of the Nucleoid-associated DNA-binding Protein H –NS with the Regulatory Region of the Osmotically Controlled proU Operon of Escherichia coli* The JOUTNAL OF BIOLOGICAL CHEMISTRY, April 1994