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Part:BBa_K2540011

Designed by: Anna Guseva   Group: iGEM18_Rice   (2018-10-06)
Revision as of 03:27, 10 October 2018 by Albert98 (Talk | contribs)


Universal Bacterial Expression Resource (UBER)

Universal Bacterial Expression Resource (UBER) is a system containing T7 RNA Polymerase (T7 RNAP) which can be used for the expression of genes under T7 promoter. UBER includes T7 RNAP and TetR repressor which provides negative feedback on the polymerase levels to help reduce toxicity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 91
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 3912


References

Kushwaha, M. & Salis, H. M. A portable expression resource for engineering cross-species genetic circuits and pathways. Nature Communications 6, 7832 (2015).

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