Regulatory

Part:BBa_R0186

Designed by: Bartholomew Canton   Group: iGEM06_MIT   (2008-04-08)
Revision as of 00:42, 9 April 2008 by Registry (Talk | contribs)

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T7 promoter (lacI repressible)

A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -9 base to an A, which should make it less than 3% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/bacteriophage/T7
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//chassis/bacteriophage/T7
Parameters
negative_regulators1
positive_regulators