Plasmid_Backbone
Part:BBa_K2862020
Designed by: Joshua Lawrence Group: iGEM18_Imperial_College (2018-10-09)
pSB1C3 for Multiple Assembly Standards (3A, BASIC and Golden Gate)
pSB1C3_BASIC (iGEM vector with BASIC sites)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 75
Illegal suffix found at 1068
Illegal SpeI site found at 151
Illegal SpeI site found at 171 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 75
Illegal NheI site found at 121
Illegal NheI site found at 144
Illegal SpeI site found at 151
Illegal SpeI site found at 171
Illegal SpeI site found at 1069
Illegal PstI site found at 1083
Illegal NotI site found at 81
Illegal NotI site found at 1076 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 75
Illegal BamHI site found at 873
Illegal XhoI site found at 2100
Illegal XhoI site found at 2992 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 75
Illegal suffix found at 1069
Illegal SpeI site found at 151
Illegal SpeI site found at 171 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 75
Plasmid lacks a suffix.
Illegal XbaI site found at 90
Illegal SpeI site found at 151
Illegal SpeI site found at 171
Illegal SpeI site found at 1069
Illegal PstI site found at 1083
Illegal AgeI site found at 734 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1054
Illegal BsaI.rc site found at 104
[edit]
Categories
Parameters
None |