Part:BBa_K2669003

Strong constitutive promotor + RBS + Optimized Amil CP (BBa_K2669002) + double terminator

Composite part containing codon optimized original native AmilCP BBa_K2669002, RBS BBa_J34801, constitutive promotor BBa_J23119 and a double terminator BBa_B0014. The part contains a blue chromoprotein which naturally exhibits strong color when expressed that can be observed with naked eye in both LB and agar culture.

The chromoprotein was first codon optimized by Austin Texas 2017 iGEM team. By performing codon optimization they hypothesized that the seemingly unstable original native amilCP from 2011 would become more stable. The Uppsala 2017 team have codon optimized the original native AmilCP further in order to get better proof of improved stability of the protein.

Part can be used as a reporter and is expressed constitutively.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]