Composite

Part:BBa_K2671000

Designed by: Oliver Hild Walett   Group: iGEM18_Linkoping_Sweden   (2018-10-02)
Revision as of 17:30, 9 October 2018 by Olle111 (Talk | contribs)


AraC-pBAD-mNG

Liu iGEM2017s plasmid with an improvement. A stop codon was mutated behind mNeonGreen (mNG). The original part was mNG fused to amyloid-β, where the fusion protein had its stop codon after amyloid-β. Another mutation was made in the GS-linker sequence where a SpeI-site was added and the sequence for amyloid-β was cleaved off. With these improvements mNG can now be used as a reporter in a fully functional operone with the pBAD-AraC inducible system. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1267
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None