Coding

Part:BBa_K2686002:Design

Designed by: Thomas Jordan   Group: iGEM18_EPFL   (2018-09-30)
Revision as of 17:01, 9 October 2018 by Caffeine4Life (Talk | contribs) (→‎Source)


Encapsulin protein with HexaHistidine insert


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 426
    Illegal SapI.rc site found at 457


Design Notes

The HexaHistidine sequence was codon optimized for bacterial expression in E. coli


Source

Derived from [[1]].

References