Device

Part:BBa_I715054

Designed by: Andrew Martens   Group: iGEM07_Davidson_Missouri_W   (2007-11-07)
Revision as of 17:41, 23 March 2008 by Anmartens (Talk | contribs) (→‎Usage and Biology)

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Test Trp anti-terminator/terminator

This part is designed to test the functionality of the trp operon regulatory sequence which uses transcriptional attenuation (via the "anti-termination" mechanism) to regulate gene expression.

Usage and Biology

In the presence of tryptophan, the trp regulatory sequence will attenuate transcription, and GFP will not be transcribed. This construct serves as a means of testing the functionality of the trp regulatory sequence by comparing GFP expression with different environmental concentrations of tryptophan amino acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1012


Functional Parameters

[edit]
Categories
Parameters
None