Composite

Part:BBa_K2557017

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)
Revision as of 15:56, 9 October 2018 by Registry (Talk | contribs)

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TetO-EF1α promoter-TP901

The TetO sequence, SV40 NLS, flag tag, EF1α promoter, and Tp901 recombinase are ligated in sequence, and participate in intracellular signal processing. According to the feedback of the digital model, the different strength promoters upstream of the recombinase and the recombination efficiency of the recombinase will affect the stability of the system. Therefore, in order to optimize our system, we used three promoters and three recombinases, hoping to find the optimal solution.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 565
    Illegal PstI site found at 1138
    Illegal PstI site found at 1849
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1138
    Illegal PstI site found at 1849
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1160
    Illegal BamHI site found at 499
    Illegal BamHI site found at 2104
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 565
    Illegal PstI site found at 1138
    Illegal PstI site found at 1849
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 565
    Illegal PstI site found at 1138
    Illegal PstI site found at 1849
    Illegal NgoMIV site found at 604
    Illegal NgoMIV site found at 748
    Illegal AgeI site found at 505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1357


[edit]
Categories
//chassis/eukaryote
Parameters
None