Composite
Part:BBa_K2557016
Designed by: Ge JT Group: iGEM18_NAU-CHINA (2018-10-09)
TetO-miniCMV promoter-TP901
The TetO sequence, SV40 NLS, flag tag, miniCMV promoter, and TP901 recombinase are ligated in sequence to participate in intracellular signal processing. According to the feedback of the digital model, the different strength promoters upstream of the recombinase and the recombination efficiency of the recombinase will affect the stability of the system. Therefore, in order to optimize our system, we used three promoters and three recombinases, hoping to find the optimal solution.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 391
Illegal PstI site found at 964
Illegal PstI site found at 1675 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 964
Illegal PstI site found at 1675 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 986
Illegal BamHI site found at 325
Illegal BamHI site found at 1930 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 391
Illegal PstI site found at 964
Illegal PstI site found at 1675 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 391
Illegal PstI site found at 964
Illegal PstI site found at 1675
Illegal NgoMIV site found at 430
Illegal NgoMIV site found at 574
Illegal AgeI site found at 331 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1183
[edit]
Categories
Parameters
//chassis/eukaryote
None |