Composite

Part:BBa_K2557011

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)
Revision as of 15:42, 9 October 2018 by Registry (Talk | contribs)

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TetO-EF1α promoter-Bxb1

The TetO sequence, SV40 NLS, flag tag, EF1α promoter, and Bxb1 recombinase are ligated in sequence, and participate in intracellular signal processing.According to the feedback of the digital model, the different strength promoters upstream of the recombinase and the recombination efficiency of the recombinase will affect the stability of the system. Therefore, in order to optimize our system, we used three promoters and three recombinases, hoping to find the optimal solution.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 565
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1279
    Illegal BamHI site found at 499
    Illegal BamHI site found at 720
    Illegal BamHI site found at 1036
    Illegal BamHI site found at 1744
    Illegal BamHI site found at 2035
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 565
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 565
    Illegal NgoMIV site found at 1021
    Illegal AgeI site found at 505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 741


[edit]
Categories
//chassis/eukaryote
Parameters
None