Composite

Part:BBa_K2557010

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)
Revision as of 15:39, 9 October 2018 by Registry (Talk | contribs)

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TetO-miniCMV promoter-Bxb1

The TetO sequence, SV40 NLS, flag tag, miniCMV promoter, Bxb1 recombinase are sequentially linked and involved in intracellular signal processing. According to the feedback of the digital model, the different strength promoters upstream of the recombinase and the recombination efficiency of the recombinase will affect the stability of the system. Therefore, in order to optimize our system, we used three promoters and three recombinases, hoping to find the optimal solution.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1099
    Illegal BamHI site found at 325
    Illegal BamHI site found at 540
    Illegal BamHI site found at 856
    Illegal BamHI site found at 1564
    Illegal BamHI site found at 1855
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 841
    Illegal AgeI site found at 331
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 561


[edit]
Categories
//chassis/eukaryote
Parameters
None