Reporter

Part:BBa_K2610017

Designed by: Charlotte de Ceuninck van Capelle, Marjolein Crooijmans   Group: iGEM18_Leiden   (2018-10-09)
Revision as of 15:12, 9 October 2018 by Registry (Talk | contribs)

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pMicF-GFP

This reporter part features the basic part promoter MicF (BBa_K2610007) and the fluorescent reporter protein GFP (BBa_E0040).

MicF small RNA, coded by the MicF gene, is produced as stress response in Escherichia coli. It negatively regulates translation of the OmpF porin. MicF binds to OmpF mRNA and represses production of OmpF in response to environmental and internal stresses. The MicF expression is stimulated by many factors such as oxidative stress, osmolarity increase and temperature increase.

More in depth information can be found on our results page.

pMicF as a non-specific stress indicator

We, iGEM Leiden 2018, have designed this composite part as part of our project Fifty Shades of Stress. This reporter part allowed us to detect stress-induced changes in MicF transcription.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 951


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Parameters
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