Coding

Part:BBa_K2557001

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)
Revision as of 15:03, 9 October 2018 by Registry (Talk | contribs)

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Bxb1 recombinase

Bxb1 is a widely studied recombinase that we use to mediate intracellular signals.In order to allow the recombinase to enter the nucleus and function, we added SV40 NLS to the front end. To detect protein content, we added the FLAG tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 774
    Illegal BamHI site found at 215
    Illegal BamHI site found at 531
    Illegal BamHI site found at 1239
    Illegal BamHI site found at 1530
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 516
    Illegal AgeI site found at 6
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 236


[edit]
Categories
//chassis/eukaryote
Parameters
None