Regulatory
Part:BBa_K2610010
Designed by: Charlotte de Ceuninck van Capelle, Marjolein Crooijmans Group: iGEM18_Leiden (2018-10-09)
pRecN
The RecA protein is required for the repair of double strand breaks in bacterial DNA. It has been shown that RecN is upregulated in E.coli after administration of known carcinogenic agent MNNG.
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the RecN promoter is not affected by the tested antibiotics.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
| None |